		Technical Data
T9160-02C	Tumor Necrosis Factor alpha, Recombinant, Canine (TNFa)	20ug

Growth Factors, Cytokines

Storage: 4°C/-70°CShipping: Blue Ice

Source:
Recombinant protein expressed in Pichia pastoris

Description:
Canine Tumor Necrosis Factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the canine inflammatory response.

Biological Activity:
This product has an ED50 of 10pg/ml, determined by measuring the cytolytic effect of recombinant canine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.

Endotoxin Level:
<1.1.EU/ug

Applications:
Suitable for use in In vitro applications and ELISA. Other applications not tested.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume and store at -20°C. Reconstituted product is stable for 12 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.3kD

Purity: >95% by SDS PAGE analysis.
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.