Technical Data
Tumor Necrosis Factor alpha, Recombinant, Equine (TNFa)
Molecular Biology Storage: 4C/-70CShipping: Blue Ice
Recombinant protein expressed in Pichia pastoris

Equine Tumour necrosis factor alpha (TNF alpha) is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.

Biological Activity:
This product has an ED50 < 0.05ng/ml, determined by measuring the cytolytic effect of recombinant equine TNF alpha on murine WEHI-13VAR-164 cells in the presence of Actinomycin D.

Endotoxin Level:

Suitable for use in In vitro applications and ELISA. Other applications not tested.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume and store at -20C. Reconstituted product is stable for 12 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: >95% by SDS PAGE analysis.
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.