		Technical Data
T9160-04K	Tumor Necrosis Factor alpha (TNFa)

Description:
Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistology (frozen and paraffin): 1:10-1:50. Non-specific background staining is seen in connective tissue.
Flow Cytometry: 1:10-1:50
Western Blot: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6A649	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Human TNFa.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

Specificity:

Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not crossreact with TNF-beta, lymphotoxin or receptor bound TNF-alpha. Species Crossreactivity: mouse, guinea pig and rhesus monkey.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Gerspach, J et al., (2000) 50:243. 2. Bradding, P et al., (1994) Am J Respir Cell Mol Biol, 10:471. 3. Radding, P et al., (1994) Clin Exp Allergy 25:406. 4. Laan van der, N et al., (2001) Arch Dermatol Res 293: 226.