Technical Data
Tumor Necrosis Factor alpha (TNFa)
Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.

Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistology (frozen): 1:50. 6um tissue sections fixed in acetone for 10 min (4).
Immunohistochemistry (paraffin): 1:50. Tissue sections were fixed in 5% formalin embedded in paraffin and cut into 2um sections. (5)
Flow Cytometry: 1:50. Cells were fixed in PBA containing 0.2% formaldyde (1).
Western Blot: 1:50 detects a band at ~17kD. A reduced sample treatment and 15% SDS-PAGE were used. (4)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG16A649Affinity Purified
100ug-20CBlue IceHumanMouse
Human TNFa.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not crossreact with TNF-beta, lymphotoxin or receptor bound TNF-alpha. Species Crossreactivity: mouse, guinea pig and rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gerspach, J et al., (2000) 50:243. 2. Bradding, P et al., (1994) Am J Respir Cell Mol Biol, 10:471. 3. Radding, P et al., (1994) Clin Exp Allergy 25:406. 4. Laan van der, N et al., (2001) Arch Dermatol Res 293: 226.