		Technical Data
T9160-10B	Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)	10ug 50ug

Growth Factors, Cytokines

Storage: -70°CShipping: Blue Ice

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF.

Endotoxin: <0.01ng/ug or <0.1EU/ug as per LAL

Biological Activity: recombinant Tumor Necrosis Factor-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 2x107IU/mg.

AA Sequence:
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.5kD

Source: Recombinant, human, E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.