		Technical Data
T9160-10C	Tumor Necrosis Factor alpha, Recombinant, Human(TNFa)	10ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Recombinant TNF alpha comprises a 157 amino acid fragment (77-233) corresponding to the mature soluble TNF alpha chain protein. TNF alpha is expressed in E. coli with an amino-terminal hexahistidine tag.

TNF alpha is a potent pleiotrophic cytokine, produced by white blood cells, endothelium and several other normal and tumor cells in response to a wide variety of stimuli. TNF alpha is involved in the pathophysiological processes of several chronic and acute diseases including the induction of apoptosis and up regulation of inflammation.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
21.85kD

Source: Human, Recombinant, E. coli
Purity: Purified. 95% by SDS-PAGE.
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in PBS, 50% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Carswell, E et al., (1975) Proc. Natl. Acad. Sci U.S.A. 72 (9): 3666-3670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.