Technical Data
Tumor Necrosis Factor alpha, Recombinant, Human(TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant TNF alpha comprises a 157 amino acid fragment (77-233) corresponding to the mature soluble TNF alpha chain protein. TNF alpha is expressed in E. coli with an amino-terminal hexahistidine tag.

TNF alpha is a potent pleiotrophic cytokine, produced by white blood cells, endothelium and several other normal and tumor cells in response to a wide variety of stimuli. TNF alpha is involved in the pathophysiological processes of several chronic and acute diseases including the induction of apoptosis and up regulation of inflammation.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Human, Recombinant, E. coli
Purity: Purified. 95% by SDS-PAGE.
Concentration: ~0.1mg/ml
Form: Supplied as a liquid in PBS, 50% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Carswell, E et al., (1975) Proc. Natl. Acad. Sci U.S.A. 72 (9): 3666-3670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.