Technical Data
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Human Tumor Necrosis Factor alpha is a single, non-glycosylated, polypeptide chain containing 158aa and having a molecular weight of 17.5kD. The sequence of the first five N-terminal amino acids was determined to be Met-Val-Arg-Ser-Ser.

Biological Activity:
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is a specific activity of 5x10e7 IU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with ddH2O, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 95% by RP-HPLC and SDS-PAGE
Form: Supplied as a lyophilized powder from 20mM PBS, pH 7.2, 100mM sodium chloride. Reconstitute with sterile ddH2O to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.