Technical Data
T9160-10E
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)
10ug
50ug
50ug
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Technical Data |
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T9160-10E |
Tumor Necrosis Factor alpha, Recombinant, Human (TNFa) |
10ug 50ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Human TNF is a 17kD factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26kD form of TNF is expressed as a membrane bound molecule. TNF is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF have been observed. Source: Escherichia coli. Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is 0.05ng/ml, corresponding to a Specific Activity of 2 x10e7 IU/mg. Endotoxin: 1EU/ug (LAL) Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months at -20°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 1 month at 4 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~17.5kD |
Source: E. coli Purity: 97% as determined by SDS-PAGE and HPLC. Form: Supplied as a lyophilized powder from 15mMNa2HPO4, 0.3M sodium chloride. Reconstitute with sterile dH2O to 0.1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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