Technical Data
TNFa, Recombinant, Human, Western Blot Control (Tumor Necrosis Factor, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFSF2)
Molecular Biology Storage: -20CShipping: Blue Ice
TNF alpha is a potent pleiotrophic cytokine, produced by white blood cells, endothelium and several other normal and tumor cells in response to a wide variety of stimuli. TNF alpha is involved in the pathophysiological processes of several chronic and acute diseases including the induction of apoptosis and up regulation of inflammation.

Recombinant corresponding to aa77-233 from human TNF alpha, fused to 6x His-tag at N-terminal, expressed in E. coli.

Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE).
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Carswell, E et al., (1975) Proc. Natl. Acad. Sci U.S.A. 72 (9): 3666-3670.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.