Technical Data
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
Molecular Biology Storage: -20CShipping: RT
Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Murine TNF-alpha is a 17.5kD protein containing 156 amino acid residues.

Biological Activity:
The ED50 was determined by the cytolysis of murine L929 cells in the presence of actinomycin D and is <0.1ng/ml, corresponding to a specific activity of >1x10e7u/mg

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 5-10mM sodium phosphate, pH 7.5. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Murine, recombinant protein expressed in E. coli.
Purity: 98% by SDS-PAGE, HPLC Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5.
Reconstitue with sterile 5-10mM sodium phosphate, pH 7.5 to a concentration of 0.1-1mg/ml. Do not vortex.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Soto, P. et al., (2003) Amer. Reproductive Immunology 50: 380-338. 2. Cherry, J.A. et al., (2008) J of Reproduction and Development 54:183-191. 3. Sullivan, B.T. et al., (2010) Int J Gynecol Cancer. 20; 1474-1481.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.