Technical Data
T9160-15
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
5ug
20ug
20ug
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Technical Data |
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T9160-15 |
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa) |
5ug 20ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Tumor Necrosis Factor-alpha (TNF-alpha) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Murine TNF-alpha is a 17.5kD protein containing 156 amino acid residues. Biological Activity: The ED50 was determined by the cytolysis of murine L929 cells in the presence of actinomycin D and is <0.1ng/ml, corresponding to a specific activity of >1x10e7u/mg Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 5-10mM sodium phosphate, pH 7.5. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 17.5kD |
Source: Murine, recombinant protein expressed in E. coli. Purity: 98% by SDS-PAGE, HPLC Endotoxin: 0.1ng/ug (1EU/ug) Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5. Reconstitue with sterile 5-10mM sodium phosphate, pH 7.5 to a concentration of 0.1-1mg/ml. Do not vortex. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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US Biological application references: 1. Soto, P. et al., (2003) Amer. Reproductive Immunology 50: 380-338. 2. Cherry, J.A. et al., (2008) J of Reproduction and Development 54:183-191. 3. Sullivan, B.T. et al., (2010) Int J Gynecol Cancer. 20; 1474-1481.
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