Technical Data
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Recombinant corresponding to mouse TNF-a expressed in E. coli.

Molecular Weight:

Biological Activity:
ED50 range=0.005-0.02ng/ml, determined by the dose dependent cytotoxicity of L929 cells. Optimal concentration for individual application should be determined by a dose response assay.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Fransen, L., et al. (1985) Nucleic Acids Res. 13(12):4417-29.2. Koh, J.S., Z.Y. Wang, and J.S. Levine (2000) J. Immunol. 165(8):4190-4201.3. Marmenout, A.,et al. Eur. J. Biochem. 152(3):515-22.4. Rott, D., et al J. Infect. Dis. 184(9):1109-1113.5. Shea-Eaton, W.K., P.P.H. Lee, and M.M. Ip (2001). Endocrinology 142(6):2558-2568.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.