Technical Data
T9160-15B
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
10ug
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Technical Data |
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T9160-15B |
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Source: Recombinant corresponding to mouse TNF-a expressed in E. coli. Molecular Weight: ~17.5kD Biological Activity: ED50 range=0.005-0.02ng/ml, determined by the dose dependent cytotoxicity of L929 cells. Optimal concentration for individual application should be determined by a dose response assay. Endotoxin: ~0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~17.5kD |
Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Fransen, L., et al. (1985) Nucleic Acids Res. 13(12):4417-29.2. Koh, J.S., Z.Y. Wang, and J.S. Levine (2000) J. Immunol. 165(8):4190-4201.3. Marmenout, A.,et al. Eur. J. Biochem. 152(3):515-22.4. Rott, D., et al J. Infect. Dis. 184(9):1109-1113.5. Shea-Eaton, W.K., P.P.H. Lee, and M.M. Ip (2001). Endocrinology 142(6):2558-2568.
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