Technical Data
T9160-15C
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)
5ug
20ug
100ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
Recombinant murine TNF-alpha produced in S. lividians is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32D.

Biological Activity: rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x10e8 IU/mg.

AA Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
17.4kD
Source: Recombinant, murine, E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder in 50mM Tris-HCL pH-8 and 20mM NaCL. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Tumor necrosis factor-alpha mediates oligodendrocyte death and delayed retinal ganglion cell loss in a mouse model of glaucoma. J Neurosci 2006 Dec 6;26(49):12633-41
2. Endogenous prostaglandins E2 and F 2alpha serve as an anti-apoptotic factor against apoptosis induced by tumor necrosis factor-alpha in mouse 3T3-L1 preadipocytes. Biosci Biotechnol Biochem 2006 Sep;70(9):2145-53
3. Toxoplasma gondii inhibits toll-like receptor 4 ligand-induced mobilization of intracellular tumor necrosis factor alpha to the surface of mouse peritoneal neutrophils. Infect Immun 2006 Jul;74(7):4274-81
4. Ursolic acid enhances cyclooxygenases and tumor necrosis factor-alpha expression in mouse skin. Biosci Biotechnol Biochem 2006 Apr;70(4):1033-7
5. The role of tumor necrosis factor alpha and the peroxisome proliferator-activated receptor alpha in modulating the effects of fumonisin in mouse liver. Toxicology 2006 May 15;222(3):165-74
6. Acute p38-mediated modulation of tetrodotoxin-resistant sodium channels in mouse sensory neurons by tumor necrosis factor-alpha. J Neurosci 2006 Jan 4;26(1):246-55

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.