Technical Data
T9160-15P
Tumor Necrosis Factor alpha, Recombinant, Porcine (TNFa)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Source:
Recombinant corresponding to porcine TNF-a expressed in E. coli.

AASequence:
LRSSSQTSDK PVAHVVANVK AEGQLQWQSG YANALLANGV KLKDNQLVVP TDGLYLIYSQ
VLFRGQGCPS TNVFLTHTIS
RIAVSYQTKV NLLSAIKSPC QRETPEGAEA KPWYEPIYLG GVFQLEKDDR LSAEINLPDY LDFAESGQVY FGIIAL

Biological Activity:
ED50 range=0.001-0.015ng/ml, determined by the dose dependent cytotoxicity of porcine PK15 cells. Optimal concentration for individual application should be determined by a dose response assay.

Specific Activity:
1.0x10e9-6.6x10e7units/mg

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~17.1kD
Purity: ~95% (SDS-PAGE). Endotoxin: 0.1ng/ug
Form: Supplied as a lyophiized powder. Carrier free.
Reconstitution: Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Bertoni, G. et al. (1993) Improved bioassay for the detection of porcine tumor necrosis factor using a homologous cell line: PK(15). J. Immunol. Meth. 160:267-271

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.