Technical Data
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha, TNFalpha, Tnfa, TNF-a, Cachectin, DIF, MGC151434, Tumor Necrosis Factor, Tnf, Tnfsf1a, Tumor Necrosis Factor Ligand Superfamily Member 2, Tnfsf2)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to 156aa of human Tumor Necrosis Factor-alpha expressed in S.Lividians is a single, non-glycosylated, polypeptide chain.

Molecular Weight:

Biological Activity:
The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg.

Dimers and Aggregates:

Protein Content:
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of TNF- as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: Not determined
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Tumor necrosis factor alpha (TNF) suppresses cAMP response element (CRE) activity and nuclear CRE binding protein in MA-10 mouse Leydig tumor cells. Endocrine 2005 Jun;27(1):17-24. 2. Increases in tumor necrosis factor-alpha following transient global cerebral ischemia do not contribute to neuron death in mouse hippocampus. J Neurochem 2005 Jun;93(6):1616-22. 3. Atrial contractile dysfunction, fibrosis, and arrhythmias in a mouse model of cardiomyopathy secondary to cardiac-specific overexpression of tumor necrosis factor-{alpha}. Am J Physiol Heart Circ Physiol 2005 Oct;289(4):H1456-67. 4. Relocation of truncated bid plays an important role in suppression of tumor necrosis factor alpha induced apoptosis in hepatocytes isolated from transgenic mouse. J Surg Res 2005 Aug;127(2):157-63. 5. Growth factors effects on preimplantation development of mouse embryos exposed to tumor necrosis factor alpha. Reprod Biol 2005 Mar;5(1):83-99. 6. Induction of epithelial cell apoptosis in the uterus by a mouse uterine ischemia-reperfusion model: possible involvement of tumor necrosis factor-alpha. Biol Reprod 2005 May;72(5):1282-8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.