Technical Data
T9160-15Q
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha, TNFalpha, Tnfa, TNF-a, Cachectin, DIF, MGC151434, Tumor Necrosis Factor, Tnf, Tnfsf1a, Tumor Necrosis Factor Ligand Superfamily Member 2, Tnfsf2)
5ug
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Technical Data |
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T9160-15Q |
Tumor Necrosis Factor alpha, Recombinant, Mouse (TNF-alpha, TNFalpha, Tnfa, TNF-a, Cachectin, DIF, MGC151434, Tumor Necrosis Factor, Tnf, Tnfsf1a, Tumor Necrosis Factor Ligand Superfamily Member 2, Tnfsf2) |
5ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to 156aa of human Tumor Necrosis Factor-alpha expressed in S.Lividians is a single, non-glycosylated, polypeptide chain. Molecular Weight: ~17.3kD Biological Activity: The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x108IU/mg. Dimmers and Aggregates: 1% (SDS-PAGE) Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of TNF- as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~17.3kD |
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) Concentration: Not determined Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Tumor necrosis factor alpha (TNF) suppresses cAMP response element (CRE) activity and nuclear CRE binding protein in MA-10 mouse Leydig tumor cells. Endocrine 2005 Jun;27(1):17-24. 2. Increases in tumor necrosis factor-alpha following transient global cerebral ischemia do not contribute to neuron death in mouse hippocampus. J Neurochem 2005 Jun;93(6):1616-22. 3. Atrial contractile dysfunction, fibrosis, and arrhythmias in a mouse model of cardiomyopathy secondary to cardiac-specific overexpression of tumor necrosis factor-{alpha}. Am J Physiol Heart Circ Physiol 2005 Oct;289(4):H1456-67. 4. Relocation of truncated bid plays an important role in suppression of tumor necrosis factor alpha induced apoptosis in hepatocytes isolated from transgenic mouse. J Surg Res 2005 Aug;127(2):157-63. 5. Growth factors effects on preimplantation development of mouse embryos exposed to tumor necrosis factor alpha. Reprod Biol 2005 Mar;5(1):83-99. 6. Induction of epithelial cell apoptosis in the uterus by a mouse uterine ischemia-reperfusion model: possible involvement of tumor necrosis factor-alpha. Biol Reprod 2005 May;72(5):1282-8.
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