Technical Data
Tumor Necrosis Factor alpha (TNFa)
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Suitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed frozen tissues
Optimal dilutions to be determined by the researcher.

Matched Pairs:
Capture: T9160-32A
Detection: T9160-18A

X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG15E852Affinity Purified
500ug4C (-20C Glycerol)Blue IceHumanMouse
Human recombinant tumor necrosis factor of alpha type.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.