Technical Data
T9160-18A
Tumor Necrosis Factor alpha (TNFa)
Description:
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed frozen tissues
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Capture: T9160-32A
Detection: T9160-18A

Hybridoma:
X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15E852Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~8mg/ml
Immunogen:
Human recombinant tumor necrosis factor of alpha type.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.