Technical Data
T9160-20A
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
10ug
|
Technical Data |
|
T9160-20A |
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa) |
10ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: RT |
|
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Source: Recombinant corresponding to rat Tumor Necrosis Factor alpha expressed in E. coli. Endotoxin: ~0.1ng/ug Biological Activity: ED50 range =0.005-0.02ng/ml, determined by the dose dependent cytolysis of mouse L-929 cells in the presence of actinomycin D. Optimal concentration for individual application should be determined by a dose response assay. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 17kD |
Source: E. coli Purity: ~95% (SDS-PAGE). Purified by sequential chromotography. Concentration: ~0.1-1mg/ml Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
1. Albina, J.E., et al., Am. J. Physiol.-Cell Physiology 281(6): C1971-C1977 (2001). 2. Balchak, S.K., et al., Reproductive Toxicology 14: 533-539 (2000). 3. Bhalla, D.K., et al., Toxicol. Sci. 69(2): 400-408 (2002). 4. Davis, J.R., et al., Am. J. Physiol.-Regulatory Integrative and Comparative Physiology 282(2): R390-R399 (2002). 5. Fang, L., et al., Am. J. Physiol.-Regulatory Integrative and Comparative Physiology 280(5): R1356-R1363 (2001). 6. Hou, S.H., et al., J. Virol. 76(7): 3212-3220 (2002). 7. Krown, K.A., et al., J. Clin. Invest. 98(12): 2854-2865 (1996). 8. Lee, S.J., et al., J. Immunol. 165: 4658-4666 (2000). 9. Lee, S.J., et al., J. Immunol. 164: 1277-1285 (2000). 10. Loparev, V., et al., Proc. Nat’l. Acad. Sci. 95(7): 3786-3791 (1998). 11. McTiernan, C.F., et al., Circ. Research 81: 493-503 (1997). 12. Miagkov, A., et al., Proc. Nat’l. Acad. Sci. 95(23): 13,859 (1998). 13. Roos, A., et al., J. Leukocyte Biol. 64: 503-510 (1998). 14. Tsai, S.-Y., et al., Cell Tissue Res. 303: 423-431 (2001). 15. Yang, S.Q., et al., Proc. Nat’l. Acad. Sci. 94: 2557-2562 (1997).
|
||