Technical Data
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Molecular Biology Storage: -70CShipping: RT
Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.

Recombinant corresponding to rat Tumor Necrosis Factor alpha expressed in E. coli.

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 range =0.005-0.02ng/ml, determined by the dose dependent cytolysis of mouse L-929 cells in the presence of actinomycin D. Optimal concentration for individual application should be determined by a dose response assay.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE). Purified by sequential chromotography.
Concentration: ~0.1-1mg/ml
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.