Technical Data
Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Rat TNF-alpha is a single, non-glycosylated, polypeptide chain containing 157aa and having a molecular weight of 17.33kD. The sequence of the first five N-terminal aa was determined to be Met-Leu-Arg-Ser-Ser. Contains less than 1% dimers and aggregates.

E. coli

Molecular Weight:

Biological Activity:
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is <0.05ng/ml, corresponding to a specific activity of 5x10e7 IU/mg.

Endotoxin: 0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 97% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Form: Supplied as a lyophilized powder. Reconstitute using sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.