Technical Data
T9160-32A
Tumor Necrosis Factor alpha (TNFa)
Description:
TNF-a is a macrophage/monocyte cytokine, usually found as a soluble secreted protein, which has diverse immunomodulatory, anti-tumor and toxic effects. Neutralizes the recombinant TNF-a mediated cytotoxicity of L929 cells and inhibits the tumor growth in mice. Protects mice against the toxicity of recombinant human TNF-a.

Applications:
Suitable for use in Immunohistochemistry, ELISA and Inhibition of biological activity of TNFa in different biological fluids. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Formalin-fixed frozen tissues
Optimal dilutions to be determined by the researcher.

Matched Pair:
Capture: T9160-32A
Detection: T9160-18A

Hybridoma:
X63.Ag8.653 myeloma cells with spleen cells of Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.T.152Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Human recombinant tumor necrosis factor of alpha type.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human TNFa. Effective in the inhibiting the biological activity of recombinant and naturtal TNFa.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.