Technical Data
Tumor Necrosis Factor beta (TNFb), Recombinant, Human
Molecular Biology Storage: -20CShipping: RT
Tumor Necrosis Factor-b (TNF-b) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-b is a 18.6kD protein containing 171 amino acid residues.

Source: E. coli

Form: Lyophilized from a sterile filtered solution in PBS, pH 7.2, 20mM sodium chloride.

Purity: 98% by SDS-PAGE and HPLC.

Endotoxin: 0.1ng/ug

Reconstitution: Reconstitute in sterile dH2O to a concentration of 0.1-1mg/ml. This solution can be diluted into other buffered solutions or stored at -20C for future use.

Recommended Dilution: 0.05-10 ng/ml for most in vitro applications.

Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin-D is < 0.05ng/ml, corresponding to a specific activity of 2x10e7 units/mg.
Responding Cells (partial list): Tumor cells, certain other cells.

Concentration Range for most in vitro applications: 0.1-10ng/ml

Country of Origin: USA

Molecular Weight:
Source: Human

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.