Technical Data
T9161-05K
Tumor Necrosis Factor Receptor 1, Recombinant, Human (Tumor Necrosis Factor Receptor Type I, TNF-RI, TNFR-I, TNFR1, TNF-R1, CD120a, p55, p60, TNFAR, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNFRSF1A)
20ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
TNFR1 belongs to the TNF-receptor superfamily. TNFR1 is a receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. There are 2 types of soluble TNF receptors: sTNFR-I and sTNFR-II, which act to neutralize the biological activities of TNF alpha and TNF beta. The levels of these soluble receptors seem to increase as a result of shedding of the extracellular domains of the membrane bound receptors. TNF-a, TNFR1 and TNFR2 have roles in cellular differentiation. TNFR1 and TNFR2 function in cell type-specific renal injury. TNFR1 is capable of signaling both cell survival and apoptosis. TNFR1-induced apoptosis requires 2 sequential signaling complexes. TNFR1 is capable of activating NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Oxidative stress promotes TNFR1 and TNFR2 self-interaction, ligand-independent and enhanced ligand-dependent TNF signaling. TNFR1 Human Recombinant produced in E. coli is a single, non-glycosylated, polypeptide chain containing 162aa and having a total molecular mass of 18.2kD. TNFR1 Human Recombinant is purified by proprietary chromatographic techniques

Source:
Recombinant corresponding to human TNFR1 expressed in E. coli.

AA Sequence:
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESSGSF TASENHLRHC LSCSKCRKEM GQVEKSSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

Molecular Weight:
~18.2kD

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18.2kD
 Source: E. coli
Purity: ~97% (RP-HPLC, SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder from 10mM sodium phosphate buffer, pH 7.5. Reconstitute with sterile dH2O to 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.