Technical Data
T9161-26E
Tumor Necrosis Factor Receptor 2, p75/p80 (TNFR2, CD120b) (Biotin)
Description:
TNF-RII is present on most cell types and is considered to be an important co-stimulator together with TNF-RI in cellular responses to TNF-alpha, in particular the transmembrane form of TNF-alpha. A dominant role of TNF-RII has been shown in thymocyte activation by TNF-alpha. The antibody is a non-agonistic receptor modulating antibody. It enhances in vitro TNF responses by increasing the affinity of the soluble form to TNF-alpha to TNF-RII.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot and ELISA.
Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:10
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Immunohistochemistry: human lymphnodes
Flow Cytometry: PHA activated T cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A665Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Human CD120b.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA. Labeled with Biotin.
Specificity:
Reacts with the extracellular part of the TNF-RII. It also reacts with the soluble receptor. Reactivity with cellbound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Grell, M et al; The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor. Cell 1995, 85: 793. 2. Grell, M et al; Tumor necrosis factor (TNF) receptor type 2 mediates thymocyte proliferation independently of TNF receptor type 1. Eur J Immunol 1998, 28: 257. 3. Grell, M et al; The type 1 receptor (CD120a) is the high affinity receptor for soluble tumor necrosis factor. Proc Natl Acad Sci USA 1998, 95: 570.