		Technical Data
T9161-56C	Tumor Necrosis Factor Receptor Associated Factor 3, CT (TRAF-3)

Description:
Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll- like receptor (IL-1/TLR) superfamily (1). TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1 (2,3). Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF- B (4). Besides CD40, TRAF3also interacts with the TRFR superfamily member lymphotoxin-b receptor (LTbR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbR (5).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000 Detects a band at 64kD.
Immunohistochemistry (paraffin sections): 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
3Y3cell lysate

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide cooresponding to aa 5-25, KKMDSPGALQTNPPLKLHTDR, of human TRAF3. (Genbank accession No. NP_663777).

Purity:

Serum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

Specificity:

Recognizes human TRAF3 and TRAF3b. Does not recognize other members of the TRAF family.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Arch RH, et al., Genes Dev. 1998; 12:2821- 30. 2. Cheng G, et al., Science 1995; 267:1494-8. 3. Mosialos G, et al., Cell 1995; 80:389- 99. 4. van Eyndhoven WGet al. Mol. Immunol. 1999; 36:647-58. 5. Kuai J, et al., J. Biol. Chem. 2003; 278:14363-9.