Technical Data
T9161-56D
Tumor Necrosis Factor Receptor Associated Factor 3, CT (TRAF-3)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry: 2ug/ml
Optimal dilution determined by the researcher.

Positive control:
3T3 cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to 16 amino acids near the carboxy terminus of human TRAF3 (Genbank accession No. NP_663777).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Reacts with human and mouse TRAF3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Arch, R.H., et al., (1998), “Tumor necrosis factor receptor-associated factors (TRAFs) – a family of adaptor proteins that regulate life and death”, Genes Dev. 12: 2821-2830
2.Cheng, G., et al., (1995), “Involvement of CRAF1, a relative of TRAF, in CD40 signaling”, Science, 267: 1494-1498
3.Mosialos G., et al., (1995), “The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family”, Cell, 80: 389-399
4.van Eyndhoven, W.G., et al., (1999), “TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0kappaB activation”, Mol. Immunol., 36: 647-658
5.Kuai, J., et al., (2003), “Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin-beta receptor reveals a novel mechanism of apoptosis”, J. Biol Chem., 278: 14363-14369