Technical Data
Tumor Necrosis Factor Receptor Associated Factor 3, CT (TRAF-3)
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry: 2ug/ml
Optimal dilution determined by the researcher.

Positive control:
3T3 cells

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PabIgGAffinity Purified
100ug-20°CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to 16 amino acids near the carboxy terminus of human TRAF3 (Genbank accession No. NP_663777).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Reacts with human and mouse TRAF3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Arch, R.H., et al., (1998), “Tumor necrosis factor receptor-associated factors (TRAFs) – a family of adaptor proteins that regulate life and death”, Genes Dev. 12: 2821-2830
2.Cheng, G., et al., (1995), “Involvement of CRAF1, a relative of TRAF, in CD40 signaling”, Science, 267: 1494-1498
3.Mosialos G., et al., (1995), “The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family”, Cell, 80: 389-399
4.van Eyndhoven, W.G., et al., (1999), “TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0kappaB activation”, Mol. Immunol., 36: 647-658
5.Kuai, J., et al., (2003), “Endogenous association of TRAF2, TRAF3, cIAP1, and Smac with lymphotoxin-beta receptor reveals a novel mechanism of apoptosis”, J. Biol Chem., 278: 14363-14369