Technical Data
T9161-59
Tumor Necrosis Factor Receptor Associated Factor 4 (TRAF-4)
Description:
The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1:TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. Human TRAF4 is a 470aa protein.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:300-1:800, transfected cells.
Immunohistochemistry (paraffin sections): 1:500-1:1000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to aa1-18 (MPGFDYKFLEKPK RRLLC) of human TRAF4. This sequence is completely conserved in mouse TRAF4.
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes human TRAF4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Krajewska, M., et al., Am. J. Pathol. 152: 1549 (1998).