Technical Data
T9161-65
Tumor Necrosis Factor Receptor Associated Factor 6, CT (TRAF-6)
Description:
TRAFs (Tumor necrosis factor receptor-associated factors) are cytoplasmic adaptor proteins that associate with tumor necrosis factor receptors (TNFR), thus mediating tumor necrosis factor (TNF) induced signaling. The TRAF family consists of six members (TRAF1-6).1-3 TRAF proteins play an important role in regulating cellular death and cellular response to stress by mediating stimulation of AP-1 family through activation of MAP kinases, including JNK/SAPKs, ERKs and p38s.4 They are also involved in the regulation of inflammatory and anti-apoptotic responses through activation of the transcription factors of NF-kB.5 TRAF6 possesses several binding motifs in CD40, TRANCE-R and IRAK suggesting a crucial role in TRANCE-R signaling. It is involved in the activation of the anti-apoptotic serine/threonine kinase Akt/PKB through TRANCE-R.6 Due to its specific receptor-binding TRAF-C domain, TRAF6 is the only member of the TRAF family that also participates in signal transduction of the interlukin-1 receptor (IL-1R)/Toll-like receptor (TLR) superfamily.1,7,8 TRAF6 plays a crucial role in adaptive immunity, innate immunity, bone homeostasis, perinatal and postnatal survival, and cytokine signaling.9

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blotting: 1-3ug/ml
Immunoprecipitation: 7ug/reaction
Optimal dilutions to be determined by the researcher.

Positive Controls:
Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~ 0.1mg/ml
Immunogen:
Synthetic peptide derived from the C-terminal region of the human and mouse TRAF6 (TNF receptor-associated factor 6) protein.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human TRAF6 proteins. On Western blots, it identifies a band at ~55kD and a band at ~60kD. The two bands may represent the two reported forms of this protein (522 and 530 amino acids). Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cao Z, et al. Nature 383:443-446, 1996. 2. Nakano H, et al. J Biol Chem 271:14661-14664, 1996. 3. Rothe M, et al. Cell 78:681-692,1994. 4. Karin M. Philos Trans R Soc Lond B Biol Sci 351:127-134, 1996 5. Darnay BG, et al. J Biol Chem 274:7724-7731, 1999. 6. Wong BR, et al. Mol Cell 4:1041-1049, 1999. 7. Aderem A and Ulevitch RJ. Nature 406:782-787, 2000. 8. Chung JY, et al. J Cell Sci 115:679-688, 2002. 9. Ye H, et al. Nature 418:443-447, 2002.