Technical Data
Tumor Necrosis Factor Receptor Associated Factor 6, CT (TRAF-6)
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB (1,2). TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families (3). TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands (4). It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) (5) or by other TLR adaptor molecules such as TRIF (6). Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK (7).

Can be used for detection of TRAF6 by Western blot at 1 to 2ug/ml.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
100ug-20CBlue IceHumanRabbit
Peptide corresponding to 14 amino acids near the C-terminus of human TRAF6 (GenBank accession no. S71821)
Purified by Ion Exchange chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human TRAF6. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 2. Wu H. Adv. Protein Chem. 2004; 68:225-79. 3. Wajant H and Scheurich P. Prog. Mol. Subcell. Biol. 2004; 34:47-72. 4. Kobayashi T, et al. Immunity 2003; 19:353-63. 5. Cao Z, Xiong J, . Nature 1996; 383:443-6. 6. Sato S, . J. Immunol. 2003; 171:4304-10. 7. Ye H, Aaron JR, Lamothe B, et al. Distinct molecular mechanism for initiating TRAF6 signaling. Nature 2002; 418:443-7.