Technical Data
T9161-66
Tumor Necrosis Factor Receptor Associated Factor 6, CT (TRAF-6)
Description:
Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-kB (1,2). TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families (3). TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands (4). It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) (5) or by other TLR adaptor molecules such as TRIF (6). Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK (7).

Applications:
Can be used for detection of TRAF6 by Western blot at 1 to 2ug/ml.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Peptide corresponding to 14 amino acids near the C-terminus of human TRAF6 (GenBank accession no. S71821)
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes human TRAF6. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 2. Wu H. Adv. Protein Chem. 2004; 68:225-79. 3. Wajant H and Scheurich P. Prog. Mol. Subcell. Biol. 2004; 34:47-72. 4. Kobayashi T, et al. Immunity 2003; 19:353-63. 5. Cao Z, Xiong J, . Nature 1996; 383:443-6. 6. Sato S, . J. Immunol. 2003; 171:4304-10. 7. Ye H, Aaron JR, Lamothe B, et al. Distinct molecular mechanism for initiating TRAF6 signaling. Nature 2002; 418:443-7.