Technical Data
Tumor Necrosis Factor Receptor Associated Factor 5 (TRAF-5)
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymhocyte activation (5).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
HeLa cell lysate can be used as positive control and an approximately 64 kD band is detected.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceHumanMouse
Fusion protein corresponding to amino acids 77 to 186 of human TRAF5
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human Tumor Necrosis Factor Receptor Associated Factor 5 (TRAF-5)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rothe M, et al. Cell 78: 681-692 (1994). 2. Cheng G, et al Science 267: 1494-1498 (1995). 3. Nakane H, et al J. Biol. Chem. 271: 14661-14664 (1996). 4. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996). 5. Nakano H, roc. Natl. Acad. Sci. USA 96 (17): 9803-9808 (1999).