Technical Data
T9161-78A
Tumor Necrosis Factor Receptor Associated Factor 5 (TRAF-5)
Description:
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymhocyte activation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16D826Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein corresponding to aa77-186 of human TRAF5 (tumor necrosis factor (TNF) receptor-associated factor 5) (O00463).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human TRAF5 at ~64kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.