Technical Data
T9162-75
Tumor Necrosis Factor Receptor 2 Soluble, Recombinant, Human (sTNFR 2)
5ug
20ug
Molecular Biology Storage: -20CShipping: Blue Ice
TNFRII is a member of the TNFR family of transmembrane proteins, and is expressed in immune cells and certain endothelial cells. It is a high affinity receptor for TNF-alpha but manifests a lower affinity to TNF-beta. Signaling through this receptor regulates various biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. Soluble TNFRII is capable of inhibiting TNF-alpha induced activities by acting as a decoy receptor. Recombinant human sTNFRII is an 18.9kD protein (174 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFRII protein.

Biological Activity:
Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25ng/ml of recombinant human TNF-alpha, is 0.125ug/ml.

Amino Acid Sequence:
MAPEPGSTCR LREYYDQTAQ MCCSKCSPGQ HAKVFCTKTS DTVCDSCEDS TYTQLWNWVP ECLSCGSRCS SDQVETQACT REQNRICTCR PGWYCALSKQ EGCRLCAPLR KCRPGFGVAR PGTETSDVVC KPCAPGTFSN TTSSTDICRP HQICNVVAIP GNASMDAVCT STSP

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
18.9kD
Source: Recombinant corresponding to 174aa from the cysteine rich ligand binding portion of the extracellular domain of the human TNFRII protein expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC. Endotoxin:
0.1ng/ug (1EU/ug)
Form: Suppllied as a lyophilized powder from 10mM sodium phosphate, pH 8.0, 50mM sodium chloride. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml. Do not vortex.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.