		Technical Data
T9300-03	TWIST1 (Twist Homolog 1, TWIST 1, TWIST-1, ACS3, bHLHa38, BPES2, BPES3, SCS, TWIST, Twist-related Protein 1)

Description:
Twist is a bHLH family transcription factor overexpressed in gliomas and a range of other cancers. It may have a role in CNS development and angiogenesis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000
Immunohistochemistry (Paraffin): 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not Determined

Immunogen:

Synthetic peptide corresponding to the N-terminal domain of human TWIST1.

Purity:

Serum

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with 200ul sterile ddH2O.

Specificity:

Recognizes human TWIST1 at ~21kD. Species Crossreactivity: mouse and rat. Does not react with Twist-2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hao H, Liu J, Liu G, et al. Depletion of GRIM-19 accelerates hepatocellular carcinoma invasion via inducing EMT and loss of contact inhibition. Journal of Cellular Physiology. 2011 Nov 21.