Technical Data
U1000-06Q
Ubiquitin (Lys63)
Description:
Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFkappaB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Inhibition and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: Precipitated ubiquitin-containing proteins from HeLa cell lysates.
Immunohistochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Immunocytochemistry: Ubiquitin (Lsy63 specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat and rabbit brain sections.
Inhibition: Preincubation of U1000-06Q with K63- multiubiquitin chains inhibited binding to HeLa cell lysate in Western Blots.
Optimal dilutions to be determined by the researcher.

Positive Control: Lys63 polyubiquitin chains

Recommended Secondary Antibodies:
I1904-46E: IgG, H&L (HRP) DkxRb
I1904-46K: IgG, H&L X-Absorbed (HRP) DkxRb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9H285Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Humanized Di-ubiquitin linked at Lys63.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin. Species Crossreactivity: mouse, rabbit and rat. Broad species cross-reactivity is expected based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Newton, K., et al. (2008) Cell 134: 668678. 2. Stewart, GS., et al. (2009) Cell 136: 420434. 3. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb1g6j/pdb 4. Ikeda, F., & Dikic. I. EMBO Reports 9:536-
542.