		Technical Data
U1000-94D	E2EPF, CT (Ubiquitin-conjugating Enzyme E2S, Ubiquitin-conjugating Enzyme E2-24kD, Ubiquitin-protein Ligase S, Ubiquitin Carrier Protein S, E2-EPF5, Ubiquitin-conjugating Enzyme E2-EPF5, UBE2S, E2EPF, OK/SW-cl.73)

Description:
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the C-terminal region of human E2EPF (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human E2EPF.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Liu, Z., et al., J. Biol. Chem. 267(22):15829-15835 (1992).