Technical Data
U2800-02C
Urotensin II (UII, U-II, Urotensin-2, UTS2, PRO1068, UNQ525/PRO1068)
Description:
Urotensin II (U-II) is a cyclic heptapeptide that functions both as a potent vasoconstrictor and a neurotransmitter/ neuromodulator. U-II circulates in the plasma as a zymogen, and is activated by the urotensin converting enzyme. U-II is the endogenous ligand for the G-protein coupled receptor 14 (GPR14), recently renamed the UT receptor. Changes in the expression of U-II and its receptor have been associated with cardiovascular and renal diseases, including atherosclerosis, myocardial infarction, and diabetic nephropathy.

Applications:
Suitable for use in ELISA and Radioimmunoassay. Other applications not tested.

Recommended Dilution:
Radioimmunoassay: 0.24ug/ml will bind ~30% in a system using human 125I-Urotensin II as a tracer.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to Glu-Thr-Pro-Asp-Cys-Phe-Trp- Lys-Tyr-Cys-Val (Disulfide Bridge: Cys5-Cys10) from human Urotensin II.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.5. Reconstitute with 200ul sterile ddH2O.
Specificity:
Recognizes human Urotensin II. Shows 69% crossreactivity with rat Urotensin II and 10% with mouse Urotensin II. Does not crossreact with Endothelin-1 (human, porcine, rat, mouse, canine or bovine), CN-22 (human, porcine, rat), rat Adrenomedullin (aa1-50), ANF (rat, rabbit, mouse), Bradykinin, human Angiotensin II, rat BNP-45, rat BNP-32 or rat PAMP-20.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.