Technical Data
U4101-37
Ancient Ubiquitous Protein 1, aa8-60 (AUP1)
Description:
AUP1 contains a domain with homology to the ancient conserved region of the archain 1 gene and a domain thay may be involved in binding ubiquitin-conjugating enzymes. The unprocessed precusor is of 476 amino acids in length and has an estimated molecular weight of 53kD.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (paraffin embedded and frozen tissues):1:500 -1:6000.
Western Blot: 1:500 -1:6000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20C. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanSheep
Concentration:
Not determined
Immunogen:
A synthetic peptide (HVFLVSCALPDSV) corresponding to the aa48-60 of human ancient ubiquitous protein 1 conjugated to blue carrier protein.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O.
Specificity:
Recognizes ancient ubiquitous protein 1. Species crossreactivity: bovine, sheep, human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wilkinson, K.D. et al. J. Biol. Chem. 256, 9235-9241, (1981) 2. Schwartz, A.L. et al. Annu. Rev. Med. 50, 57-74, (1999) 3. Coux, O., et al. Annu. Rev. Biochem. 65, 801-847, (1996) 4. Strausberg R.L, et al. Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). 5. Jang W, et al. Genomics 36:366-368(1996). 6. Shibata K, et al. Genome Res. 10:1757-1771(2000). 7. Carninci P, et al. Genome Res. 10:1617-1630(2000). 8. Kawai J, et al. Nature 409:685-690(2001). 9. Okazaki Y, et al. ature 420:563-573(2002). 10. Carninci P, et al. Science 309:1559-1563(2005).