Technical Data
V2024-01A
VAMP 1,2 (Vesicle-Associated Membrane Protein, Synaptobrevin)
Description:
Vesicle-associated membrane protein (VAMP), also known as synaptobrevin, is an 18kD integral membrane protein localized to the cytoplasmic surface of synaptic vesicle (2). VAMP consists of a proline-rich amino-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a carboxyl-terminal tail (3). Two VAMP homologs, VAMP1 and VAMP2, are differentially expressed in the nervous system (3). VAMP1 expression is localized to neurons involved in modulating overlapping patterns, whereas VAMP2 is found in neurons associated with autonomic, sensory and integrative functions. In non-neuronal tissues, VAMP1 is restricted to pancreas and kidney tubular cells, whereas VAMP2 is predominantly expressed in Langerhans islets and glomerular cells (4). Evidences for VAMPs involvement in vesicular transport and neurotransmission include the requirement of its yeast homologs, SNC 1 and 2, in targeting and fusion of Golgi- derived vesicles with the plasma membrane (5). VAMP is also the target of clostridial neurotoxins that block neurotransmitter release from presynaptic terminals (6). VAMP is a component of a 20S complex implicated in vesicle docking and fusion. The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, alpha-SNAP, and NSF (7).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot (Colorimetric): 1:4000
Immunohistochemistry: 1:50 on human neuroblastoma tissue.
Optimal dilutions to be determined by researcher.

Positive Controls:
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM4E240Purified
SizeStorageShippingSourceHost
25ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Immunoprecipitated synaptic vesicle-containing fractions from human brain homogenates.
Purity:
Purified by ammonium sulfate pecipitation.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Specificity:
Recognizes human VAMP on SDS-PAGE immunoblots at ~18kD. Recognizes the VAMP1 aa1-118 and the VAMP2 aa33-96-GST fusion proteins expressed in E. coli. No reactivity was seen with a ~18kD protein in mouse liver extracts. Species Crossreactivity: mouse, rat, bovine, hamster, porcine and Xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Honer, W.G., et al., Brain Rsch. 609: 9-20 (1993). 2. Calakos, N. & Scheller, R., Physiological Reviews 76: 1-29 (1996). 3. Trimble, W.S., et al., J. Neurosci. 10: 1380-1387 (1990). 4. Rossetto, O., et al., J. Cell Biology 132: 167-179 (1996). 5. Protopopov, V., et al., Cell 74: 855-861 (1993). 6. Schiavo, G., et al., Nature 359: 832-835 (1992). 7. Sollner, T., et al., Cell 75: 409-418 (1993). 8. Ferrer-Montiel, A.V., et al., FEBS Lett. 435: 84-88 (1998). 9. Cho, S.J., et al., Proc. Natl. Acad. Sci. USA 99(7): 4720-4724 (2002).