Technical Data
V2024-75
VAMP 7 (Vesicle-associated Membrane Protein 7, VAMP7, VAMP-7, HGNC:11486, Synaptobrevin-like 1, SYBL1, Tetanus-insensitive VAMP, TI VAMP)
Description:
Tetanus Insensitive VAMP protein (Ti-VAMP)is a v-SNARE (soluble N-ethylmaleimide-sensitive fusion protein [NSF] attachment protein [SNAP] receptor), molecule involved in specific intracellular transports to the apical plasma membrane in epithelial cells, a tetanus neurotoxin-resistant pathway. Various combinations of SNARE proteins play an essential role in diverse intracellular membrane fusion pathways, including exocytosis. Tetanus neurotoxin-insensitive VAMP (TI-VAMP/VAMP-7) has been reported to function in some regulatory and constitutive exocytosis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:2000
Immunohistochemistry: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
MabIgG1,k9F457Serum
SizeStorageShippingSourceHost
200ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
TI-VAMP recombinant protein.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 200ul sterile ddH2O.
Specificity:
Recognizes human VAMP 7 at ~25kD. Species Crossreactivity: mouse, rat and canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Muzerelle A et al; Neuroscience. 2003;122:59-75