Technical Data
V2100-25B
Varicella Zoster Virus, Ellen Strain (VZV, VZVgE, VZVgI, Envelope Glycoprotein gI, GI, Glycoprotein IV, GPIV, Human Herpes Virus 3, Human Herpes virus 3, HHV3, Membrane Glycoprotein gE)
Description:
Varicella Zoster Virus (VZV), a member of the human herpes virus family, causes two distinct clinical manifestations: childhood chickenpox(Varicella) and shingles (zoster). Varicella is the outcome of the primary infection with VZV, whereas, zoster is the result of VZV reactivation from latently infected sensory ganglia which occurs predominantly in aging and immunosuppressed individuals. VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV, however there is no equivalent of HSV gD. VZV virons are spherical and 150-200nm in diameter. Its lipid envelope encloses the nucleocapsid of 162 capsomeres arranged in a hexagonal form. Its DNA is a single linear, double strand molecule, 125,000nt long. Glycoprotein E (gE), a 623aa type I integral membrane protein, is expressed on plasma membranes and in the cytoplasm of VZV infected cells, where it is presumed to be present in the membranes of intracellular vesicles or organelles. VZV gE complexes with gI in the rough endoplasmic reticulum, and most gE exists in the form of gE to gI heterodimers in infected cells. VZV gE is also a predominant component of the virion envelope. The functions of gE, which is encoded by ORF68, are of particular interest because it has been demonstrated that ORF68 is essential for VZV replication. In contrast, the genes for gE proteins can be deleted from herpes simplex virus and pseudorabies virus, albeit with significant reductions in infectivity in cell culture and in animal models. Since the VZV genome does not encode a homologue of gD, VZV gE may have functions that are usually segregated between gD and gE, or the gE to gI complex, in other alphaherpesviruses.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 1:10-1:500
Immunocytochemistry/Immunofluorescence: 1:10-1:2,000
Immunohistochemistry: 1:10-1:2,000
Western Blot: 1`:100-1:2,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19F469Affinity Purified
SizeStorageShippingSourceHost
125ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Varicella Zoster Virus Ellen Strain. Cellular Localization: Cell membrane, cytoplasmic
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 20mM sodium phosphate, pH 9.0. No preservatives added.
Specificity:
Recognizes the Varicella Zoster Virus, Ellen Strain. Reacts with the carboxy region of the Varicella Zoster Virus. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Davison AJ et al. New common nomenclature for glycoprotein genes of varicella-zoster virus and their glycosylated products. J Virol 57:1195-7 (1986). Drew WL & Mintz L Rapid diagnosis of varicella-zoster virus infection by direct immunofluorescence. Am J Clin Pathol 73:699-701 (1980).