Technical Data
V2109-52
Vascular Endothelial Cadherin (VE-Cadherin, CD144)
Description:
Human vascular endothelium cadherin is an endothelium-specific cadherin localized at the intercellular junctions. The antibody can be used to investigate the functional role of VE-cadherin.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot, Confocal microscopy and Functional applications. Not suitable for use in paraffin sections. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, Formalin or acetone-fixed): 1ug/ml Not suitable for use in paraffin sections.
Western Blot: 1ug/ml
Functional Applications: Interferes with VE-cadherin adhesion (reversible endothelium disruption). This effect mimics the transmigration of leukocytes or cancer cells through endothelium.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corresponding to aa1-258 of human VE-cadherin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.3.
Specificity:
Recognizes human VE-cadherin. Interferes with VE-cadherin adhesion (reversible endothelium disruption), an effect which mimics the transmigration of leukocytes or cancer cells through endothelium. Species Crossreactivity: mouse, rat and bovine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gulino, D., et al., J. Biol. Chem. 273: 29,786 (1998). 2. Iyer, S., et al., Am. J. Physiol. Lung Cell Mol. Physiol. 286: L1143 (2004).