		Technical Data
V2110	Vascular Endothelial Growth Factor, Recombinant, Human (VEGF)	2ug 10ug

Growth Factors, Cytokines

Storage: -20°CShipping: RT

Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2kD protein consisting of two 165 amino acid polypeptide chains.

AA Sequence: APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Best if stored at -20°C. Reconstituted VEGF should be stored in working aliquots at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
38.2kD

Source: Human, recombinant protein expressed in E. coli
Purity: 98% by SDS-PAGE and HPLC
Endotoxin: 0.1ng/ug (1Eu/ug)
Form: Supplied as a lyophilized powder without additives.
Reconstitution: Reconstitute in sterile dH2O to 0.1-1mg/ml.
Specificity: Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-8ng/ml.
Responding Cells (partial list): Endothelial cells

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Myoken, Y., et al., Proc. Natl. Acad. Sci. USA 88: 5819-5823 (1991).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.