		Technical Data
V2110-04B	Vascular Endothelial Growth Factor B, aa22-207, Recombinant, Human (VEGFB, VEGF-B, VEGF-related Factor, VRF)	50ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

VEGF-B, also known as Vascular endothelial growth factor-B, is one of 5 mammalian VEGF family members, the others being VEGF-A, VEGF-C, VEGF-D, and placental growth factor (PlGF). VEGF-B binds to one of the VEGF receptor tyrosine kinases, namely VEGF receptor-1(VEGFR1, Flt1), but not to VEGFR2 and VEGFR3. VEGF-B is homodimeric, heparin-binding glycoprotein involved in embryonic angiogenesis and vasculogenesis. Recombinant VEGF-B protein was expressed in E. coli and purified by using conventional chromatography techniques.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA

Biological Activity:
Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 fro this effect is <1ng/ml

Storage and Stability:
Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
21.6kD

Source: E. coli
Purity: 90% (SDS-PAGE) Endotoxin: 1.0EU/1ug (LAL).
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Claesson-Welsh L. (2008) Arterioscler Thromb Vasc Biol. 28(9):1575-6. 2. Malik AK,, et al. (2006) Blood. 107(2):550-7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.