Technical Data
V2110-04H
Vascular Endothelial Growth Factor, Recombinant, Human, Glycosylated Dimer, Trimer (VEGF) (Human 293 Cells)
5ug
Growth Factors, Cytokines Storage: -20°CShipping: Blue Ice
Expression of proteins in our Human 293-cell expression system is a cost-effective and scalable production method for high-authenticity, recombinant human proteins.

Functional activities of human proteins depend on proper folding, phosphorylation, disulfide bridge formation, and proteolytic processing or appropriate glycosylation. Ideally, recombinant human proteins are produced in human cells that are more authentic in terms of both physical properties and biochemical functions. However, the current process of human cell expression requires a large quantity of DNA and medium supplemented with bovine serum. Hence, it is often a daunting task to produce sufficient amount of high quality proteins for drug discovery or diagnostic development (1-100mg) at acceptable cost. United States Biological is now supplying a high yield, cost-effective and versatile system to produce active recombinant human proteins in a human cell line to meet the special needs of the broad scientific community and drug discovery industry.

Endotoxin-free VEGF121 is expressed in human 293 cells as a glycosylated cytokine with an apparent molecular mass of 37kD as dimer and 50kD as trimer, glycosylated.

Endotoxin: 1EU/ug

Activity:
The specific activity was determined by the dose-dependent stimulation of the proliferation
of Human Umbilical Vein Endothelial Cells.

Specific Activity:
~2-8ng/ml ED50

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS, 0.1% endotoxin-free recombinant HSA (A1327-54A). Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
37kD
 Source: Recombinant Human VEGF121 expressed in human 293 cells.
Purity: 95% as determined by SDS-PAGE and Coomassie blue staining.
Form: Supplied as a lyophilized powder from TBS. Reconstitute with sterile PBS, 0.1% endotoxin-free recombinant HSA (A1327-54A).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.