Technical Data
V2110-05
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Description:
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms- like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co- operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:50-1:500. Use standard deparaffinization techniques on tissue speciens. Preheat tissues using a citrate buffer, pH 6.0 and high heat epitope retrieval techniques. Note: Do not allow tissues to dry out during the staining procedure.
Optimal dilutions to be determined by the researcher.

Control:
Pancrea, Kidney, Adenocarcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3F401Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human VEGF121 (NP_003376).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4.
Specificity:
Recognizes 34-54kD isoforms of human Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF). Species Crossreactivity: rabbit, mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mukhopadhyay, D., et al., Nature 375: 577581, 1995. 2. Shalaby, F., et al., Nature 376: 6266, 1995. 3. Fong, G.H., et al., Nature 376: 6670, 1995. 4. Cohen, T., et al., J. Biol. Chem. 270: 1132211326, 1995. 5. Yoshinari, M., Proc. Natl. Acad. Sci. USA 88: 58195823, 1991.