Technical Data
V2110-05D
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Description:
Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Human VEGF is a 38.2kD protein consisting of two 165 amino acid polypeptide chains.

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1.25-5ug/ml. Use 100ul/well for coating
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Capture: V2110-05D Vascular Endothelial Growth Factor (VEGF)
Detection: V2110-03E Vascular Endothelial Growth Factor (VEGF) (Biotin)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15J63Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant human VEGF.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Specificity:
Recognizes natural and recombinant human VEGF.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bachtiary, B., et al. (2002) Serum VEGF levels in patients undergoing primary radiotherapy for cervical cancer: impact on progression-free survival. Cancer Lett. 179(2):197-203. 2. Blann, A.D., et al. (2001) Increased serum VEGF in 13 children with Wilms' tumour falls after surgery but rising levels predict poor prognosis. Cancer Lett. 173(2):183-186. 3. Ferrara, N., et al. (2000) VEGF: an update on biological and therapeutic aspects. Curr. Opin. Biotechnol. 11(6):617-624. 4. Tischer, E., et al. (1991) The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing. J. Biol. Chem. 266(18):11947-11954.