Technical Data
V2110-06A
Vascular Endothelial Growth Factor, Endocrine-gland-Derived, Recombinant, Human (EG-VEGF)
5ug
20ug
Growth Factors, Cytokines Storage: -20°CShipping: RT
Human endocrine gland derived vascular endothelial growth factor (EG-VEGF) is selectively expressed in steroidogenic glands and promotes growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor (SF)-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility.

Recombinant Human EG-VEGF is a single, non-glycosylated, polypeptide chain containing 86 amino acids and having a molecular mass of 9.6kD.

Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Ile-Thr-Gly.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of EG-VEGF as a Reference Standard.

Biological Activity:
The biological activity is determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-5ng/ml.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, 0.1% BSA or HSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
 Source: Recombinant, Human (E. coli).
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug)
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder with no additives. Reconstitute with sterile ddH2O, 0.1% BSA or HSA. to a concentration of >0.1mg/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bv8 and endocrine gland-derived vascular endothelial growth factor stimulate hematopoiesis and hematopoietic cell mobilization. LeCouter J, Zlot C, Peale F, Proc Natl Acad Sci U S A 2004 Nov 30;101(48):16813-8 2. Differential expression of the angiogenic factor genes vascular endothelial growth factor (VEGF) and endocrine gland-derived VEGF in normal and polycystic human ovaries. Ferrara N, Frantz G, Dillard-Telm L, Peale F, Am J Pathol 2003 Jun;162(6):1881-93 3. Mouse endocrine gland-derived vascular endothelial growth factor: a distinct expression pattern from its human ortholog suggests different roles as a regulator of organ-specific angiogenesis. LeCouter J, Lin R, Zhang Z, Endocrinology 2003 Jun;144(6):2606-16 4. The endocrine-gland-derived VEGF homologue Bv8 promotes angiogenesis in the testis: Localization of Bv8 receptors to endothelial cells. LeCouter J, Lin R, Frantz G, Proc Natl Acad Sci U S A 2003 Mar 4;100(5):2685-90 5. Expression of endocrine gland-derived vascular endothelial growth factor in ovarian carcinoma. Zhang L, Yang N, Katsaros D, Toll A, Clin Cancer Res 2003 Jan;9(1):264-72 6. Endocrine gland vascular endothelial growth factor (EG-VEGF) and the hypothesis of tissue-specific regulation of angiogenesis. Ferrara N, LeCouter J, Endocr Res 2002 Nov;28(4):763-4

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.