Technical Data
V2110-07M
Vascular Endothelial Growth Factor, Recombinant, Rat (VEGF)
10ug
Molecular Biology Storage: -20CShipping: RT
VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF 165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Source:
Recombinant corresponding to 164aa from rat Vascular Endothelial Growth Factor expressed in
E.coli.

Molecular Weight:
~38.4kD

Biological Activity:
ED50 range=10-100ng/ml, determined by the dose dependent proliferation of human umbilical vein cells (HUVEC).

Endotoxin:
0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O or buffer, 0.1% BSA., aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: ~95% (SDS-PAGE). Purified by Ion Exchange chromatography.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile, ddH2O or buffer, 0.1% BSA. to a concentration of 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Barleon, B., et al. (1997) Mapping of the sites for ligand binding and receptor dimerization at the extracellular domain of the vascular endothelial growth factor receptor flt-1. J. Biol. Chem. 272:10382-10388.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.