Technical Data
V2110-08D
Vascular Endothelial Growth Factor (VEGF, VEGF 121, VEGF121, VEGF A, VEGFA, VEGF-A, MGC70609, Vascular Permeability Factor, VPF)
Description:
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.

Applications:
Suitable for use in Western Blot and Immunohistochemistry . Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.

Positive Control: VEGF 165 recombinant protein.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k9F475Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human VEGF from a glioma.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Specificity:
Recognizes human VEGF at ~19-22kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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