Technical Data
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF), involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability, inducing properties specific for the vascular endothelium. There are various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor
(VEGF/VPF) containing 206-, 189-, 165-, and 121-amino acid residues. These different species, with apparently similar biological activities (in vitro), arise from alternative splicing of the RNA transcript from a single gene. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two
(VEGF189 and VEGF206) remain cell associated.

Sutiable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/Paraffin): 5-10ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 min.

Positive Control:

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
500ul-20°CBlue IceHumanRabbit
Human recombinant VEGF165. Cellular Localization: Cytoplasmic, cell surface and extracellular matrix. MW of Antigen: 19-22kD (reduced)
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also supplied without BSA and sodium azide. Use Cat # V2110-11X.
Recognizes human Vascular Endothelial Growth Factor (VEGF). Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: García-Gómez, I. et al., (2005) Neurological Research, 27: 807-811. 1. Int. J. Oncology 8: 505-511 (1996). 2. Boocock, C.A., et al., J. of National Cancer Inst. 87(7): 506-516 (1995). 3. Brown, L.F., et al., Human Pathology 26(1): 86-91 (1995).