Technical Data
V2110-13
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Description:
VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Applications:
Suitable for use in Neutralization of the bioactivity of human VEGF. Other applications have not been tested.

Recommended Dilution:
Neutralization: Azide removal is required prior to use.
Optimal dilutions to be determined by the researcher.

Positive Control:
Angiosarcoma

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.N.600Affinity Purified
SizeStorageShippingSourceHost
500ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant human VEGF121. Cellular Localization: Cytoplasmic, cell surface and extracellular matrix. MW of Antigen: 19-22kD (reduced)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Available without BSA and sodium azide.
Specificity:
Recognizes human Vascular Endothelial Growth Factor (VEGF). Epitope has not been determined. Species Crossreactivity: Rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bobcock, C.A., et al., Journal of the National Cancer Institute 87(7): 506-516 (1995). 2. Brown, L.F., et al., Human Pathology 26(1): 86-91 (1995).