		Technical Data
V2110-13	VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

Description:
VEGF (Vascular Endothelial Growth Factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeablility inducing properties specific for the vascular endothelium. Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell-associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

Applications:
Suitable for use in Neutralization of the bioactivity of human VEGF. Other applications have not been tested.

Recommended Dilution:
Neutralization: Azide removal is required prior to use.
Optimal dilutions to be determined by the researcher.

Positive Control:
Angiosarcoma

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	0.N.600	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Recombinant human VEGF121. Cellular Localization: Cytoplasmic, cell surface and extracellular matrix. MW of Antigen: 19-22kD (reduced)

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Available without BSA and sodium azide.

Specificity:

Recognizes human Vascular Endothelial Growth Factor (VEGF). Epitope has not been determined. Species Crossreactivity: Rabbit.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bobcock, C.A., et al., Journal of the National Cancer Institute 87(7): 506-516 (1995). 2. Brown, L.F., et al., Human Pathology 26(1): 86-91 (1995).