		Technical Data
V2110-13B	VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)

Description:
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Angiosarcoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab			Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.2mg/ml

Immunogen:

Synthetic peptide derived from N-terminal of human VEGF.Cellular Localization: Cytoplasmic, cell surface, extracellular matrix

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.

Specificity:

Recognizes human VEGF and the 165, 189 and 121 amino acid splice variants.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.