Technical Data
V2110-13B
VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF)
Description:
VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml for 2 hours at RT
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Angiosarcoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide derived from N-terminal of human VEGF. Cellular Localization: Cytoplasmic, cell surface, extracellular matrix
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Specificity:
Recognizes human VEGF and the 165, 189 and 121 amino acid splice variants.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.