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Technical Data |
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V2110-15 |
Vascular Endothelial Growth Factor, Human (VEGF) BioAssay™ ELISA Kit |
1Kit |
| Kits and Assays | Storage: -20°CShipping: Blue Ice |
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Vascular Endothelial Growth Factor, Human (VEGF) BioAssay™ ELISA Kit contains the key components required for the quantitative measurement of natural and/or recombinant hVEGF in a sandwich ELISA format within the range of 63-2000pg/ml. Using the ELISA protocol provided, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay hVEGF ~1000 ELISA plate wells. Specificity: When tested at 50ng/ml the following antigens exhibited 100% crossreactivity: Human VEGF121, Murine VEGF When tested at 50ng/ml the following antigens exhibited <1% crossreactivity: Human: EG-VEGF, EGF, RANTES When tested at 50ng/ml the following antigens did not exhibited significant crossreactivity: Human: FGF-16, GM-SCF, PDGF-AA, PDGF-AB, PDGF-BB, SCF. Murine: EGF, GM-CSF, SCF. Kit Components: V2110-15A1: Capture Antibody, 1x50ug V2110-15A2: Detection Antibody, 1x100ug V2110-15A3: Human Recombinant VEGF Standard, 1x1ug V2110-15A4: Avidin Peroxidase Conjugate, 1x60ul Storage and Stability: Store kit at -20°C. Stable for 6 months at -20°C. *Note: Store V2110-15A4: Upon receipt, aliquot into ten 6ul vials. Store at -20°C for up to 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Kisliouk, T.: Presence and regulation of endocrine gland vascular endothelial growth factor/prokineticin-1 and its receptors in ovarian cells. Journal of Clinical Endocrinolology and Metabolism 88(8): 3700-7 (2003).
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