Technical Data
V2110-15
Vascular Endothelial Growth Factor, Human (VEGF) BioAssay™ ELISA Kit
1Kit
Kits and Assays Storage: -20°CShipping: Blue Ice
Vascular Endothelial Growth Factor, Human (VEGF) BioAssay™ ELISA Kit contains the key components required for the quantitative measurement of natural and/or recombinant hVEGF in a sandwich ELISA format within the range of 63-2000pg/ml. Using the ELISA protocol provided, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay hVEGF ~1000 ELISA plate wells.

Specificity:
When tested at 50ng/ml the following antigens exhibited 100% crossreactivity: Human VEGF121, Murine VEGF
When tested at 50ng/ml the following antigens exhibited <1% crossreactivity:
Human: EG-VEGF, EGF, RANTES
When tested at 50ng/ml the following antigens did not exhibited significant crossreactivity:
Human: FGF-16, GM-SCF, PDGF-AA, PDGF-AB, PDGF-BB, SCF.
Murine: EGF, GM-CSF, SCF.

Kit Components:
V2110-15A1: Capture Antibody, 1x50ug
V2110-15A2: Detection Antibody, 1x100ug
V2110-15A3: Human Recombinant VEGF Standard, 1x1ug
V2110-15A4: Avidin Peroxidase Conjugate, 1x60ul

Storage and Stability:
Store kit at -20°C. Stable for 6 months at -20°C. *Note: Store V2110-15A4:
Upon receipt, aliquot into ten 6ul vials. Store at -20°C for up to 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kisliouk, T.: Presence and regulation of endocrine gland vascular endothelial growth factor/prokineticin-1 and its receptors in ovarian cells. Journal of Clinical Endocrinolology and Metabolism 88(8): 3700-7 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.