Technical Data
Vascular Endothelial Growth Factor 165, Recombinant, Human (VEGF 165)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human VEGF165 (GenBank Accession No. NM_001025368) is a 38.2kD disulfide-linked homodimeric protein (aa207-371), consisting of two 165 amino acid polypeptides.

Recombinant human VEGF165 expressed in E. coli.

Amino Acide Sequence:

Biological Activity:
ED50 is < ng/ml as determined by the dose-dependent stimulation
of the proliferation of human umbilical vein endothelial cells (HUVEC).

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with 4mM HCl. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC) Endotoxin: 1EU/ug (LAL).
Form: Supplied as a lyophilized powder from 20mM HOAc. No preservatives added. Reconstitute with 4mM HCl to a concentration of 0.1-1.0mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. VEGF serum levels are not associated with Parkinson's disease Eur. J. Neurol. 14 (8), E6 (2007). 2. Association between vascular endothelial growth factor gene polymorphisms and survival in hepatocellular carcinoma patients Hepatology 46 (2), 446-455 (2007).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.